Description

Location: Room 1.20B, MRC Building

Advantages: Large range of objective lens magnifications and resolutions, high sensitivity, large field of view, multiple fluorescence filters available, Perfect Focus for long term live imaging

Disadvantages: Large images lead to very large file sizes so data may need to be binned or cropped, too slow for high-speed Z series

GFP3 consists of a Nikon Ti inverted microscope stand with LED light sources for fluorescence illumination (CoolLED pE-300) and transmitted light illumination (pE-100), motorised stage and a Nikon DS-Qi2 high sensitivity scientific CMOS camera with a 1608 x 1608 pixel chip to capture images of large areas. There are a range of objective lenses, including a high resolution 60x oil lens. All are capable of phase contrast as well as fluorescence imaging. Several fluorescent filter sets are available for the microscope; the standard sets include DAPI, GFP, Texas Red and Cy5. There are also sets for CFP and YFP. Not all the sets will necessarily be installed on the microscope at the same time because there are only six positions in the reflector turret. Please ask a member of light microscopy staff if you need to use a filter that is not currently installed.