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MICROSCOPE: Nikon N-STORM based on ECLIPSE Ti-E inverted microscope stand



STORM RESOLUTION 

XY

~ 20 - 30 nm

Z

~ 50 - 75 nm

3D STORM axial range

± 500 nm

A


IMAGING MODES

  • STORM (2D or 3D using cylindrical lens)
  • Multiparameter widefield with laser or LED illumination (multi-colour, multi-point, time-lapse)

  • TIRF


LIGHT SOURCES

Light Source

Type

Wavelengths [nm]

Nikon LU-NV laser unit

Laser

405, 488, 561, 647

Lumencor SPECTRA Light Engine® 

LED

395, 440, 470, 508, 555, 640

A



OBJECTIVE LENSES

Objective

Magnification

Immersion Medium

Numerical Aperture

Coverslip Correction [mm]

Working Distance [mm]

CFI Plan Fluor 10x

10x

air

0.3

0.17

16

CFI Plan Fluor 40x oil

40x

oil

1.3

0.17

0.2

CFI Plan Apo VC 60x WI

60x

water

1.2

0.15 – 0.18

0.31 – 0.28

CFI Plan Apo VC 60x oil

60x

oil

1.4

0.17

0.13

CFI Apo TIRF 100X Oil

100x

oil

1.49

0.13 – 0.19 (23°C)

0.14 (37°C)

0.12

A



FLUORESCENCE FILTER SETS 

Turret Position

Emission Filter

Example Fluorophores

1

49000

DAPI

2

49002

GFP

3

49008

mCherry

4

C-S2

Quadband compatible with all Lumencore lines

5

C-STO

Quadband compatible with all Agilent laser lines

6

N-STO

Alexa Fluor 647, Cy5

A


CAMERAS

  • Andor iXon DU897E (EMCCD) for STORM imaging (right side port)

  • Andor Technology sCMOS Zyla 4.2 for widefield acquisitions


INCUBATION: Solent Scientific chamber heater and CO2 supply unit


SOFTWARE: NIS-Elements Ar including NIS-A N-STORM analysis module


ADDITIONAL INFORMATION

  • anti-vibration optical table
  • fully enclosed 
  • perfect focus system (PFS)
  • motorized XY stage with encoders
  • piezo Z stage
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