Radiance
Confocal laser scanning microscope
This is a Bio-Rad Radiance confocal microscope mounted on a Nikon E800 manual upright microscope stand. There are three lasers with six laser lines but there is no 405 nm laser, so you cannot image DAPI or Hoechst. The confocal has three GaAsP detectors for high sensitivity imaging of poorly fluorescent specimens or at low laser power. FRAP and other photo-stimulation experiments are possible
Training & Booking
Specifications
Objective Lenses
| Objective Lenses | |||||
| Magnification | Type | Immersion | NA | DIC | WD (mm) |
|---|---|---|---|---|---|
| 10x | Plan Fluor | Air | 0.3 | - | 16 |
| 20x | Fluor | Water | 0.5 | M | 2 |
| 40x | Fluor | Water | 0.8 | M | 2 |
| 60x | Fluor | Water | 1.0 | H | 2 |
The letters in column 5 indicate the correct prism for use with the lens | |||||
Lasers
| Line | Laser | Typical Fluorophores | Notes |
|---|---|---|---|
| 458 | Argon | CFP | This is not a very powerful laser line |
| 476 | Argon | - | Could be used as an alternative for green dyes when trying to separate dyes with similar spectra |
| 488 | Argon | EGFP, FITC, Alexa 488 | |
| 514 | Argon | YFP | Could also be used for Citrine, Venus, etc |
| 543 | GreenHeNe | TRITC, Alexa 532, Alexa 546, Alexa 555, Cy3 | Can also be used for 'redder' dyes like Texas Red, Alexa 568, Alexa 594, mCherry but with lower excitation efficiency |
| 637 | Red Diode | Cy5, Alexa 633, Alexa 647, Si Rhodamine dyes, DRAQ5 |
Note that there is no 405 nm laser on this machine so you cannot image Hoechst, DAPI or anything else in the blue region of the visible spectrum