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  • Zeiss ELYRA PS.1 - Technical Specifications

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Two 6-position emission filter turrets are available for imaging on the Zeiss ELYRA PS.1, one with single-bandpass filters and another one with multi-bandpass filters.   

 

Single-band emission filter set 

Turret position

Emission filter

Excitation filter

Example Fluorophores

1

Transmission light

N/A

N/A

2

Triple-band filter (labelled None)

N/A

most common fluorophores in the visible range

3

BP 420 - 480/LP 750

MBS 405

DAPI

4

BP 495 - 550/LP 750

MBS -488

GFP, Alexa Fluor 488

5

BP 470 - 620/LP 750

MBS -561

mCherry, Texas Red

6

LP 655

MBS -642

Cy5, Alexa Fluor 647

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CAMERA/DETECTOR   

Camera

Pixels

Pixel Size [µm]

Quantum Efficiency (QE)

Andor iXon 897 (EMCCD) for PALM imaging (left side port)

512 x 512

16 x 16

90%

pco.egde sCMOS for SIM imaging (base port)

1280 x 1280

6.5 x 6.5

57%, dynamic range 15 bit

A

 

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INCUBATION

The microscope is fully enclosed in a blacked out chamber and equipped with a TokaiHit unit (INU series, Model: ELY).

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SOFTWARE

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ZEN Black Version 11.0.2.190

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ADDITIONAL INFORMATION

  • Z drive DC servomotor (smallest Z step size 25 nm)

  • motorized XY piezo scanning stage motorized (range 130 mm x 100 mm, max. sped 100 mm/s, step resolution 0.2 µm)

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  • Z-piezo stage insert for XY scanning stage (max. travel range 100 µm, min. 5 nm step size)  
  • stage inserts available for standard slides, glass-bottom dishes and LabTek chambers

  • definite focus
  • EPI, HILO and TIRF illumination mode
  • image reconstruction algorithms for SIM and PALM data