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Use a widefield microscope if your specimen is thin and relatively free of out-of focus blur; or you don't need to optically section it for processing, analysis and visualisation in 3D.

Microscope Name

Upright or Inverted?

Fluorescence Colours

Key Features

Techniques

Axioplan

Upright

Blue, Green, Red, Far-Red

Zeiss Axioplan 2 microscope

. QImaging QClick camera

. Fluorescence

. DIC.

High sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slides

Axio Imager

Upright

Blue, Green, Red, Far-Red. Cyan and Yellow available on request

Zeiss Axio Imager A1 microscope

. QImaging Retiga EXi camera

. Fluorescence

. Phase contrast

High sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides

Axioskop 3

Upright

Blue, Green, Red

Zeiss Axioskop microscope

. QImaging QICAM colour camera

. Fluorescence

. Phase contrast

. Motorised stage

Moderate to high resolution fluorescence microscopy and imaging of histologically or histochemically stained specimens on slides. Motorised stage supports tiling and stitching of specimens.

Leica

Inverted

Blue, Green, Red

Leica DMIRB microscope

. Hamamatsu Orca NRB camera

. Fluorescence

. Phase contrast

. Long distance lenses suitable for use with normal TC dishes and plates

Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides, or in dishes and plates with glass or plastic bottoms.

Leica 2

Inverted

Blue, Cyan, Green, Red

Leica DMIRB microscope

. Leica DFC7000T colour camera

. Fluorescence

. Phase contrast

. Leica Modulation Contrast (LMC)

. Long distance lenses suitable for use with normal TC dishes and plates

Moderate to high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides, or in dishes and plates with glass or plastic bottoms. Leica Modulation Contrast (LMC) lenses for brightfield contrast imaging that is less sensitive to the meniscus in well plates. Cyan filter cube for CFP.

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