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titleSuper-Resolution Facility

SuRF Access Form

Super-resolution microscope booking rules

UCL Super-Resolution Imaging Website

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titleDownloads

Sample Preparation - Pedro Pereira

STED Microscopy - Siân Culley

Optical Aberrations - Ricardo Henriques

Principles of Fluorescence Microscopy - Andrew Vaughan

Principles of Fluorescence Microscopy (Notes) - Andrew Vaughan

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titleLinks

Ricardo Henriques Group

Super-Resolution Background Information

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The Super-Resolution Facility (SuRF) at the MRC Laboratory for Molecular Cell Biology (LMCB) houses three commercial super-resolution microscopes covering a range of super-resolution techniques, including Structured Illumination Microscopy (SIM), localization microscopy (PALM/STORM) and Stimulated Emission Depletion (STED) microscopy. Super-resolution microscopy allows the imaging of fluorescently labelled probes at a resolution of just tens of nano-meters, surpassing classic light microscopy by at least one order of magnitude. Recent advances such as the development of photo-switchable fluorophores, high-sensitivity microscopes and single-molecule localisation algorithms make super-resolution imaging rapidly accessible to the wider life sciences research community. 
 

Available super-resolution microscopes:

Analysis computers for the processing of super-resolution images recorded using the Leica TCS SP8 STED 3X (ORAC) or Zeiss ELYRA PS.1 (ZEN) are located in room 2.19A. 

If you would like to use any of the microscopes within the SuRF for your research please apply for facility access by submitting the completed online application form, which can be accessed via the link on the left or here...