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  • Zeiss ELYRA PS.1 - Technical Specifications

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OBJECTIVE LENSES

Objective

Magnification

Immersion Medium

Numerical Aperture

Coverslip correction [mm]

Working Distance [mm]

EC Plan-Neofluar

10x

air

0.3

0.17

5.2

LCI Plan-Neofluar Imm Korr DIC M27 

25x

water

0.8

adjustable, 0 - 0.17

0.21 at cover glass 0.17

EC Plan-Neofluar OIL DIC M27

40x

oil

1.3

0.17

0.21

C-Apochromat Korr M27

63x

water

1.2

adjustable, 0.14 - 0.19

0.28 at cover glass 0.17

Plan-Apochromat DIC M27

63x

oil

1.4

0.17

0.19

alpha Plan-Apochromat DIC M27 Elyra

100x

oil

1.46

0.17

0.11

A

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FLUORESCENCE FILTER SETS

Two 6-position emission filter turrets are available for imaging on the Zeiss ELYRA PS.1, one with single-bandpass filters and another one with multi-bandpass filters.

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Single-band emission filter set 

Turret position

Emission filter

Excitation filter

Example Fluorophores

1

Transmission light

N/A

N/A

2

Triple-band filter (labelled None)

N/A

most common fluorophores in the visible range

3

BP 420 - 480/LP 750

MBS 405

DAPI

4

BP 495 - 550/LP 750

MBS -488

GFP, Alexa Fluor 488

5

BP 470 - 620/LP 750

MBS -561

mCherry, Texas Red

6

LP 655

MBS -642

Cy5, Alexa Fluor 647

Multi-band emission filter set   

Turret position

Emission filter

Excitation filter

Example Fluorophores

1

Empty

N/A

N/A

2

Empty

N/A

N/A

3

BP 420 - 480/BP 570 - 640/LP 740

MBS 405/561

DAPI & mCherry

4

BP 420 - 480/BP 495 - 550/LP 650

MBS 405/488/642

DAPI, GFP & Cy5

5

LBF -561/642

MBS -561/642

Alexa Fluor 555 & Alexa Fluor 647

6

LBF -488/561

MBS 405ac/488/561

DAPI, GFP & mCherry

A

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CAMERA/DETECTOR   

Camera

Pixels

Pixel Size [µm]

Quantum Efficiency (QE)

Andor iXon 897 (EMCCD) for PALM imaging (left side port)

512 x 512

16 x 16

90%

pco.egde sCMOS for SIM imaging (base port)

1280 x 1280

6.5 x 6.5

57%, dynamic range 15 bit

A

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INCUBATION

The microscope is fully enclosed in a blacked out chamber and equipped with a TokaiHit unit (INU series, Model: ELY).

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SOFTWARE

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ZEN Black Version 11.0.2.190

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ADDITIONAL INFORMATION

  • Z drive DC servomotor (smallest Z step size 25 nm)

  • motorized XY piezo scanning stage motorized (range 130 mm x 100 mm, max. sped 100 mm/s, step resolution 0.2 µm)

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  • Z-piezo stage insert for XY scanning stage (max. travel range 100 µm, min. 5 nm step size)  
  • stage inserts available for standard slides, glass-bottom dishes and LabTek chambers

  • definite focus
  • EPI, HILO and TIRF illumination mode
  • image reconstruction algorithms for SIM and PALM data